diploma in ecotoxicology

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Evaluation of the utilization of energetic reserves (lipids, sugars) as biomarkers for the effect of dinoseb on the soil invertebrate Folsomia candida (Arthropleona, Isotomidae)

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  • Objectives
  • General presentation
  • How can we evaluate the ecological toxicity and risks?
  • Biomarkerrs
  • Why do we study biomarkers on invertebrates?
  • Presentation of springtails and F candida
  • Choice of the pollutant used within the framework of this work
  1. PART I

    MATERIAL AND METHOD FOR THE REPRODUCTION TEST

    • Breeding and farming technique of the collemboles (ISO/FDIS 11267)

    • Determination of the effects of the substrate and the yeast contaminated with dinoseb on the reproduction of F candida

    RESULTS AND DISCUSSION ON THE REPRODUCTION TEST

      • Contamination of the substrate
      • Contamination of yeast
      • Summary tables for the various values obtained
      • Validation of the results
  2. PART II

    MATERIAL AND METHOD FOR THE TESTS ON ENERGETIC RESERVES

      • Method of culture and breeding of springtails
      • Contamination of the substrate
      • Setting of springtails in contact with the substrate contaminated with dinoseb
      • Counting and weighing of surviving springtails, extraction and proportioning of lipids of F candida

      • Extraction and measurement of sugars in F. candida

    RESULTS AND DISCUSSION ON MORTALITY, WEIGHT, AND RESERVES OF LIPIDS AND SUGARS IN F. CANDIDA

      • Mortality
      • Weight
      • Lipids
      • Sugars
  3. GENERAL DISCUSSION AND OUTLINES
      • Energetic reserves in F candida
      • Relation between dinoseb-organism
      • Comparison of the NOEC, LOEC, EC20 and EC50 for the two tests carried out
      • Conclusion and outlooks
  4. GLOSSARY AND ABBREVIATIONS
  5. BIBLIOGRAPHY
  6. PROTOCOLS FOR THE REPRODUCTION TEST
      • Procedure for breeding of springtails
      • Procedure for the contamination of the artificial soil and the yeast
      • Procedure for the realization of the artificial ground
      • Procedure for the isolation of organisms aged from 10 to 12 days

      • Realization of the reproduction test

    APPENDICES QUOTED IN THE TEXT

      • Méthod of Litchfield-Wilcoxon
  7. PROTOCOLS FOR THE TESTS ON ENERGETIC RESERVES
      • Breeding of the springtails
      • Contamination of the artificial soil
      • Realization of the artificial soil
      • Isolation of organisms aged from 10 to 12 days
      • Realization of the substrate and compressing of the substrate in the pots
      • Transfer of the individuals in the pots
      • Procedure for the extraction and the proportioning of lipids

      • Procedure for the extraction and the proportioning of sugars

    APPENDICES QUOTED IN THE TEXT

      • Mortality
      • Weight
      • Lipids
      • Sugars
      • general discussion
  8. METHODS Of DATA ANALYSIS
      • Methods used to compare various sets of data
      • Methods used to calculate the EC50 (or EC20) and their confidence intervals
  9. ROUGH RESULTS
  10. LIST OF TABLES AND FIGURES

Curriculum vitae

A. Margand, 2001: "Evaluation de l'utilisation des réserves énergétiques (lipides, sucres) en tant que biomarqueurs pour l'effet du dinosèbe sur l'invertébré du sol Folsomia candida (Arthropleona, Isotomidae)", travail de diplôme, Faculté des Sciences, Université de Genève, Suisse.